Beilstein J. Org. Chem.2017,13, 1099–1105, doi:10.3762/bjoc.13.109
using fluorescence microscopy, grazing-incidence angle X-ray diffraction, and infrared reflection–absorption spectroscopy. An increase of the lateral membranepressure leads to a well-packed layer of the ‘flipper’ mechanophores and a clear change in hue above 18 mN/m. The fluorescent probes had no
influence on the measured isotherm of the natural phospholipid DPPC suggesting that the flippers probe the lateral membranepressure without physically changing it. This makes the flipper probes a truly useful addition to the membrane probe toolbox.
Keywords: fluorescent probes; membrane biophysics
; membranepressure; membrane probes; monolayers; Introduction
Physical triggers are a major regulator of biological processes. The lateral bilayer membranepressure, e.g., influences the nucleation [1] and shape changes [2] of lipid domains, it gates mechanosensitive pores [3] and globally organizes cell
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Graphical Abstract
Figure 1:
Structure of the (bis)dithienothiophene mechanosensitive flipper probe. Twisted out of planarity by...